Fig. 6. Changes in pHi (expressed as pHi) (A) and in [Ca²⁺]_i (expressed as % mean of basal values) (B) of trout hepatocytes following addition of 20 mmol l^–1 NH₄Cl. pHi measurements were performed using Ca²⁺-containing medium, Ca²⁺-free medium and Ca²⁺-free medium after incubation of cells with BAPTA-AM, while [Ca²⁺]_i measurements were performed using Ca²⁺-containing and Ca²⁺-free media. Data are means ± s.e.m. of 45–58 cells in A and 24–38 cells in B from three independent preparations.