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Figure 4


Fig. 4. Spatial expression pattern of TFoxK1-{alpha} in T. rubripes embryos during development at 18°C. TFoxK1-{alpha} transcripts were detected by whole-mount in situ hybridisation with a DIG-labelled cRNA probe. The different developmental stages are (A) 57 h.p.f., bud stage of gastrulation, (B) 63 h.p.f., 5-somite stage, (C) 76 h.p.f., 10-somite stage, (D,E) 86 h.p.f., 16-somite stage. A sense (negative) control of a 10-somite stage embryo is shown in F. Anterior is top. Scale bar, 0.5 mm. h, tubular heart; hb, hindbrain; l, lens; mb, midbrain; ov, optic vesicle; psm, pre-somitic mesoderm; r, retina; rmf, rhombo-mesencephalic fissure; s, somites; tb, tail bud.