Fig. 6. Tissue-specific and head-independent phosphorylation of p38 MAPK at 0°C. (A) Red-eye stage nondiapausing pharate adults were exposed at 0°C for 2 h, and specific tissues were dissected. Tissue proteins were analyzed using western blotting with anti-phospho-p38 and anti-total p38 MAPK antibodies. (B) Red-eye stage nondiapausing pharate adults were ligated between the head and thorax with a nylon thread and exposed at 0°C. Protein samples from the posterior part were analyzed using western blotting with anti-phospho-p38 and anti-total p38 MAPK antibodies. NT, no treatment; 0°C, held at 0°C for 2 h.