Fig. 5. Phosphorylation of p38 MAPK in diapause-programmed flies reared at 20°C and exposed to 0°C. (A) Feeding 3rd instar to day of pupariation, (B) diapausing pupae 0 to 50 days after pupariation and (C) day 20 diapause pupae to which 5 µl hexane was applied to elicit diapause termination. Larvae or pupae were held at 0°C for 2 h, and protein samples were prepared. Whole body proteins were analyzed using western blotting with anti-phospho-p38 and anti-total p38 MAPK antibodies. FL, 3^rd-instar feeding larvae; W0–3, day 0–3 wandering 3^rd-instar larvae; P0–50, day 0–50 after pupariation; NT, no treatment; 0°C, held at 0°C for 2 h. Red-eye stage nondiapausing pharate adults reared at 25°C were used as controls.