Fig. 3. Temporal profiles of activation and decay of p38 MAPK phosphorylation in red-eye stage nondiapausing pharate adults. (A) Exposure to 0°C for various durations. (B) Exposure to 0°C for 10 min and then transferred to 25°C. (C) Exposure to 0°C for 2 h and then transferred to 25 and –10°C for 2 h, or held for an additional 2 h at 0°C. (D) Exposure to 0°C for 2 h and then transferred to 25°C for various durations. Flies held at 25°C were used as controls. Fly proteins were analyzed using western blotting with anti-phospho-p38 and anti-total p38 MAPK antibodies.