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Figure 4


Fig. 4. Effect of 20-hydroxyecdysone (20E) injection on hemolymph ecdysteroid concentration. Intact land crabs were injected with 20E or vehicle (10% ethanol) and sampled at 4 h, 8 h, 12 h and 24 h after injection. Ecdysteroid was quantified with radioimmunoassay. 20E caused a large, transient increase in ecdysteroid concentration in the hemolymph (values are mean ± 1 s.e.m., N=10).