Fig. 6. (A) Genistein in vitro blocks nuclear translocation of putative OREBP. The drug prevents both the translocation of OREBP and the associated increase in D2 activity. Notice that even in those explants that were not challenged, genistein reduced both protein–DNA binding and enzyme activity. Lower portion of A shows the corresponding quantification (relatively intensity) of the complexes. (B) The associated D2 activity (means ± s.e.m. of three separate experiments, each in triplicate). Normalized D2 results are expressed in arbitrary units.