Fig. 2. In vivo time course of nuclear recruitment of a putative osmotic response element binding protein (OREBP) after hypo-osmotic stress. (A) Translocation (EMSA, using ORE1 and ORE2 oligonucleotides) of putative OREBPs into the nuclei, and (B) their corresponding disappearance from the cytoplasmic compartment. Protein–DNA binding occurred in a biphasic mode: an initial protein translocation 2 h after hypo-osmotic stress, and a second and more intense wave of recruitment 8 h post-challenge. Panels show representative gels from 4–6 replicates. Below each gel is the corresponding quantification (relative intensity; arbitrary units) of the complexes. Notice the difference in the scales for nuclei (A) and cytoplasmic (B) graphs. Values are means ± s.e.m. of three separate quantifications. ^*P<0.05.