Fig. 2. Daily fecundity for constant (A) and transient (B) temperature treatments. The mean total number of eggs laid by a female varied with constant temperature treatment (A, inset; different lowercase letters indicate significant differences between treatment groups from Tukey HSD post-hoc comparisons). For the transient treatment groups (B), flies were kept at 25°C except for either 1 or 3 days at 18°C or 1 day at 29°C. The transient temperature data is presented as the percent difference from the control group maintained at constant 25°C. Each point is the mean ± s.e.m. from 15 females (range 13–17) per temperature per day, and points are slightly offset to make error bars visible. P-values from Kruskal–Wallis rank sum tests for each day are shown below each plot; values in bold type are significant at the P=0.05 level. Flies were 3–4 days old on day 1.