Fig. 3. (A) Schematic protocol of the dishabituation experiment. Each night, two introductory trials (not shown) were terminated after 30 s of suppression of male calling by the initial ticking stimuli. The top dark gray bar shows the temporal extent of the stimulus, with either a continuation or a switch to another stimulus (broken line, test trial). Lower solid gray bars show the relative timing of male advertisement and subsequent calling. The second bar becomes broken to show the time during which habituated advertisement calling was evaluated for post-stimulus-switch change, or dishabituation. ICI, interclick interval; TTH, time to habituation; TTS, time to suppression. (B) Example of a habituated bout of advertisement calling under a test condition. The male had been suppressed for approximately 4 min prior to habituation. The arrow marks the time point that the 180 ms ICI stimulus was changed to the 219 ms ICI. The bout was followed by 30 s of silence (not shown) while the 219 ms ICI stimulus continued to play. The loudest slow-trill clicks are clipped because of the male moving close to the hydrophone. On this time scale, the peaks of the fast-trill clicks cannot be easily separated by eye because the clicks overlap.