Fig. 1. The isolated brain stem of the shark Iago omanensis. (A) Careful
removal of the cartilage skull completely exposed the brain stem (white
circle), the cerebellum (green circle) and the cervical spinal cord (yellow
circle). (B) Top view of the shark's isolated brain stem. The black and yellow
lines outline the DON and the dorsal granular ridge (DGR), respectively. (C)
Side view of the shark's isolated brain stem where the DON is marked by black
line. (D) Schematic drawing of the isolated preparation, showing the relative
locations of the stimulating and the recording electrodes. PF, parallel
fibers; Aff, afferent nerve. Scale bars in A–C, 1 mm.