Fig. 2. Thermolimit respirometry and activity data from a single fly during a temperature ramp. The CO₂ output profile (in black) and the activity tracing (in red) of an LN male are shown. The black arrow indicates CT_max for spiracular control, whereas the red arrow indicates CT_max for locomotor function. The respirometry tracing shows the typical phases seen in other insect species: (A) exponential phase, (B) plateau, (C) mortal fall and (D) postmortal peak.