Fig. 8. Effect of 9-AC. (Ai) Representative recordings from one fibre performed 3 min (control), 8 min (9-AC) and 17 min after impaling the muscle fibre. 9-AC, at –120 mV, blocks I_Cl,H in a time-dependent manner and reduces the tail current on jumping back to –50 mV. Prolonged washing led to almost full recovery of I_Cl,H. (Aii) Means and their standard errors of normalized I_Cl,H from four experiments. (B) The effect of 1 mmol l^–1 9-AC on the resting membrane conductance measured by voltage ramps. 9-AC significantly lowered the membrane conductance of muscle fibres by approximately 20%. (C) Synaptic currents were consistently decreased by 1 mmol l^–1 9-AC to approximately 70% of the control value (N=4). This effect was reversed by washing with saline.