Fig. 5. Steady-state activation of the chloride-conductance, G_Cl,H. (A) Mean G–V relationship at two different internal Cl^– concentrations. Paired measurements of currents and of E_Cl were performed in six fibres after moderate and strong Cl^– loading of the fibre (the respective recordings were performed 1 and 5 min after impalement; for details of the approach and for the voltage-clamp protocols employed, cf. Materials and methods). Currents were converted to conductance, G. The conductance is greatly enhanced at raised [Cl^–]_i. This effect is largely because of the increase in maximal conductance. (B) Normalised G–V relationships for the data shown in A. The rise in [Cl]_i led to some positive shift of the curve on the voltage axis. Curves in A and B are Boltzmann-fits. For further analysis, cf. Table 2. Error bars indicate s.e.m.