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Figure 10


Fig. 10. RT-PCR analysis of the expression of ClC channels in Drosophila melanogaster muscle preparations. (A) RT-PCR analysis of body wall RNA preparations. Two independent PCR reactions were performed for each Drosophila ClC channel (primers F1-B1 in left lane and F2-B2 in right lane). (B) DmClC-a RT-PCR analysis (primers F2-B2) of five different pure muscle RNA preparations, Mu1-5 and CNS. Grey arrows indicate RT-PCR products; white arrows indicate PCR products amplified from genomic DNA; *, an unspecific amplification (as verified by DNA sequencing). DNA marker: M1, {lambda}DNA/Eco47I; M2, pBR322 DNA/BsuRI; M3, 100 bp ladder; WP, water control. `DmClC-a' corresponds to `DmClC-2', which is chiefly used in the text and which has been introduced by Flores et al. (Flores et al., 2006). (C) Localisation of the ClC-2 channel in larval longitudinal muscles by immunohistochemistry showed distinct bands of antibody staining. ClC-2-positive staining corresponds to the Z-line of the sarcomere.