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Figure 2


Fig. 2. Multiple alignment of translated amino acid sequences of Callinectes sapidus CasCAg and CasCAc isoforms (GenBank EF375491 and GenBank EF375490, respectively) with partial CA isoforms sequences from the crab Carcinus maenas (CamCA1 and CamCA2) (Henry et al., 2003) and with other well-characterized {alpha}-CA isoforms published in GenBank from invertebrates such as the insects Aedes aegypti (AeaCA: AF395662) and Anopheles gambiae (AngCA: DQ518576) and from vertebrates such as the batrachia Xenopus laevis (XelCA: BC042287), the agnatha Petromyzon marinus (DQ157849), the teleostei Tribolodon hakonensis (TrhCA: AB055617) and the mammal Homo sapiens (HosCAI: M33987; HosCAII: NM000067; HosCAIII: NM005181; HosCAIV: NM000717; HosCAV: L19297; HosCAVI: NM001215; HosCAVII: BC033865). Intensity of shading indicates degree of similarity: dark background: 100% agreement; medium dark background: >50% agreement; light background: >30% agreement. Symbols under sequences: + indicate the 36 putative active site residues; z indicates the zinc-binding histidine residues; black circles localize the active-site hydrogen bond network; and the most common proton shuttle group is boxed (Hewett-Emmett and Tashian, 1996).