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Figure 4


Fig. 4. Changes in mean cellular haemoglobin concentration (MCHC) as a function of time in air-equilibrated whole blood at 1% CO2 (A) or 3% CO2 (B) or air-equilibrated washed red blood cells (RBCs) in the presence of 10–4 mol l-1 ouabain (C). The dotted lines indicate osmotic shrinkage by addition of sucrose (calculated to yield 25% shrinkage). Control cells ({square}; N=9, 4 and 11 in A, B and C, respectively), shrunken cells ({blacksquare}; N=9, 4 and 11 in A, B and C, respectively), shrunken cells treated with 10–4 mol l-1 amiloride (Figure 4; N=9, 4 and 11 for A, B and C, respectively) or with 10–5 mol l-1 bumetanide (Figure 4; N=4, 4 and 6 in A, B and C, respectively). * indicates mean values that are statistically different from the respective control value at time zero; {dagger} indicates mean values that are statistically different from shrinkage alone; {ddagger} indicates values that are not statistically different from corresponding values in control blood samples. P<0.05.