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Figure 3


Fig. 3. Changes in mean cellular haemoglobin concentration (MCHC) as a function of time in washed red blood cells (RBCs) in the presence of 10–4 mol l-1 ouabain. The dotted line indicates osmotic shrinkage of the RBCs following addition of sucrose (calculated to yield 25% shrinkage). (A) Oxygenated control ({square}) and shrunken ({blacksquare}) as well as deoxygenated control ({circ}) and shrunken (•) RBCs. (B) Difference in MCHC between shrunken and control cells under oxygenated ({blacksquare}) or deoxygenated (•) conditions. The dashed and the dash-dot-dashed lines indicate 50% recovery from initial change in MCHC upon shrinkage in oxygenated and deoxygenated RBCs, respectively. (C) Deoxygenated control and shrunken RBCs with and without transport inhibitors. Control cells ({circ}), shrunken cells (•), shrunken cells treated with 10–4 mol l-1 amiloride (Figure 3) or with 10–5 mol l-1 bumetanide (Figure 3). * indicates mean values that are statistically different from the respective time zero value; {dagger} indicates mean values that are statistically different from shrinkage under deoxygenated conditions; {ddagger} indicates values for deoxygenated control RBCs that are statistically different from values for oxygenated control RBCs. N=5, P<0.05.