Fig. 1. Na influx in washed red blood cells (RBCs) equilibrated to a range of
oxygen partial pressure (PO2) values in the
presence of 104 mol l-1 ouabain. The influx was
measured in unstimulated cells to get basal influx (
) and in cells
stimulated by a calculated 25% osmotic shrinkage alone (
) or in the
presence of 104 mol l-1 EIPA (). The cell
suspensions were exposed to the experimental gas mixture 10 min before
shrinkage (time zero in the figures). (A) 515 min, (B) 1530 min,
(C) 3045 min and (D) 4560 min. * indicates
statistical difference from basal influx within the same
PO2 and time interval;
indicates statistical difference from air-equilibrated RBCs within the same
treatment and time interval;
indicates statistical
difference from the same treatment and PO2
level in the 515 min time interval. N=4,
P<0.05.