Fig. 3. Western blots showing the effect of superoxide dismutase (SOD) and catalase (CAT) on CoCl₂-induced phosphorylation of p38-MAPK (Ai), MAPKAPK2 (Aii) and Hsp27 (Aiii) after CoCl₂ treatment. The phosphorylated forms of the kinases were detected in extracts (50 µg of protein) from control hearts (control) or hearts perfused for 15 min with 500 µmol l^-1 of CoCl₂ in the presence or absence of SOD (12.5 U ml^-1) and CAT (150 U ml^-1). The effect of SOD and CAT alone were also assessed. An anti-actin antibody was used as a control for equal protein loading (Aiv). Densitometric analysis of phospho-p38 (B), phospho-MAPKAPK2 (C) and phospho-Hsp27 (D) was performed by laser scanning. Western blots shown are representative of at least three independent experiments; data are means ± s.e.m. for at least three independent experiments. ^*P<0.001 vs control (untreated) hearts; P<0.001 vs CoCl₂-treated hearts.