Fig. 3. Effect of prolactin (PRL), thyroid hormone (T₃) or insulin-like growth factor-1 (IGF-1) on the expression of Na⁺/K⁺-ATPase, Na⁺/K⁺/2Cl^– cotransporter (NKCC) and Na⁺/H⁺ exchanger-1 (NHE-1) in pavement cells (PVCs) in culture. The cells were exposed to the respective hormones for 24 h. Cell lysates were collected for western blot analysis (left) and the amounts of the respective ion transporter proteins were quantified (right). (A) PRL at high dose stimulated the expression of NKCC and NHE-1. (B) IGF-1 elicited a dose-dependent induction of NKCC. (C) T₃ had no observable effect to the expression of the ion transporters. *P<0.05 compared with the control. The results were obtained from more than six independent experiments.