Fig. 6. Quantitative PCR analysis of mRNA encoding C and D forms of Na⁺/K⁺-ATPase -subunit in individual gills of Pachygrapsus marmoratus transferred from 36 seawater to 10 (A,B) or to 45 (C,D) seawater. Values are mean ± s.d. (N=3 measurements at each time point using gills pooled from three or four animals). Relative expression levels are indicated in relation to a standard reference (one sample of gill 6 at 48 h after transfer to 10). Significant differences from zero time values (animals in 36 seawater) are indicated: *P<0.001; P<0.01; P<0.05.