Fig. 5. Quantitative PCR analysis of arginine kinase mRNA abundance in individual gills of Pachygrapsus marmoratus transferred from 36 to 10 (A) or to 45 (B) seawater for time periods indicated above the figure. Mean ± s.d. (N=3 or 4 measurements at each time point using gills pooled from three or four animals). Relative expression levels are indicated in relation to a standard reference (one sample of gill 6 at 48 h after transfer to 10). Significant differences from zero time values (36 seawater) are indicated: *P<0.001; P<0.01; P<0.05.