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Figure 2


Fig. 2. Complete nucleotide and predicted amino acid sequence of the D form (accession no. DQ173924) of Na+/K+-ATPase {alpha}-subunit cDNA amplified from gills of Pachygrapsus marmoratus. The C form (acc. no. DQ173925) lacks the 81-nucleotide segment and 27-amino-acid segment indicated in blue. A consensus 14-3-3 binding site RTDSYR (Aitken, 2002), indicated by a rectangle, is found near the N terminus of the D form but not the C form. In-frame stop codons are indicated in red. Ten transmembrane domains predicted by transmembrane hidden Markov modeling (TMHMM) (Sonnhammer et al., 1998) are underlined. Prosite (release 20.3) analysis identified the amino acid sequence DKTGTLT at position 390-396 (bold typeface) as a characteristic P-type ATPase motif including the aspartate that becomes phosphorylated during the transport cycle (Kaplan, 2002). A cAMP- and cGMP-dependent protein kinase phosphorylation site (RRNS) is predicted at position 954-957 (bold typeface). Brackets enclose the 707-nucleotide section initially amplified and aligned in Fig. 1 with corresponding segments from other species.