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Figure 6


Fig. 6. (A) Schematic representation of a sagittal section of the bullfrog brainstem showing the site where the rostral medullary Raphé was stimulated and level where analysis of the 5-HT immunoreactivity was performed. (B) Representative photomicrographs of cross sections comparing 5-HT immunoreactivity in the medullary Raphé (level of cranial nerve VII) of each stage group. Scale bar, 100 µm. (C) Box plots showing the number of 5-HT-immunoreactive neurons counted within the rostral medullary Raphé in the region where the stimulations were performed. The bottom of the box shows the 25th percentile and the top shows the 75th percentile. The line within the box shows the median. * Indicates a value statistically different from pre-metamorphic tadpoles; {dagger} indicates a value statistically different from the metamorphic data at P<0.05.