Fig. 8. Effects of 200 mmol l^–1 tetraethylammonium (TEA) on tuning and sensitivity evaluated from recordings of the auditory nerve of the cicada T. josei. (A) Averaged and (B) example tuning curves measured before and after drug application, and the effect of repeatedly washing with insect saline. TEA resulted in a downward shift of the characteristic frequency and a reduced sensitivity. Sensitivity and tuning were reestablished after repeatedly washing the preparation with insect saline for up to 2 h 30 min. Error bars in A indicate the standard deviation. Recordings were made at ambient temperature of 24–28°C.