Fig. 4. Effects of body temperature on tuning, sensitivity, latency and response strength of auditory interneurons of the cicada T. josei. (A,B) Tuning curves of two interneurons exhibiting shifts in their tuning and sensitivity with body temperatures ranging from 16°C to 28°C. Maximum effects are observed at temperatures from 16–18°C to 24°C in the frequency range 3 to 8 kHz. At higher frequencies the characteristic frequency remains constant, but some effect on sensitivity is still present. (C) Sensitivity at the characteristic frequency of 20 recordings, of the 29 cells listed in Table 1, which exhibited an increased sensitivity with temperature. The lines connect the sensitivities of each interneuron. (D) Dependence of latency on temperature, obtained from 29 recordings of interneurons. Latencies were measured 20 dB above threshold at the characteristic frequency and decreased with increasing temperature. (E) Dependence of the number of action potentials on the temperature in 29 recordings. At each temperature the number of action potentials is an average of five stimulus presentations at the characteristic frequency and 20 dB above threshold.