Fig. 7. Upper: representative digitized computer images of in situ hybridization of ECaC antisense probe in Procambarus clarkii antennal gland sections in various molting stages. Abundance of mRNA is illustrated by increasing yellow/orange intensity compared with control (blue). Lower: representative digitized computer images of in situ hybridization of ECaC sense probe in Procambarus clarkii antennal gland sections in various molting stages. Middle: structural regions of the antennal gland (scale bar, 100 µm), including bladder (B), coelomosac (C), labyrinth (L) and nephridial canal (NC).