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Figure 6


Fig. 6. (A) Real-time PCR assay for the expression of crayfish Procambarus clarkii ECaC mRNA in antennal gland at different molting stages. Relative quantification (RQ expressed as mean ± s.d. from three different samples with 4-5 crayfish in each sample) was performed by normalizing the Ct value of each sample with the Ct value of the endogenous control (18s rRNA gene, {Delta}Ct) and finally calculated using {Delta}Ct of control (intermolt) as calibrator. (B) Quantitative RT-PCR assay of crayfish Procambarus clarkii ECaC (upper panel) and 18s rRNA (lower panel) in antennal gland at different stages of molting cycle.