Fig. 2. The alignment of the deduced crayfish Procambarus clarkii ECaC protein sequence with pufferfish ECaC (GenBank accession number AY232821), rainbow trout ECaC (AY256348), human ECAC1 (AJ271207), rabbit ECaC (AJ133128) and rat CaT1 (AF160798). Amino acids are numbered on the right and significant residue identities are indicated by an asterisk (*). Light-gray boxes indicate ankyrin repeat domains, dark-gray boxes are predicted transmembrane domains and the black box is the putative pore-forming region. The putative protein kinase A and C phosphorylation sites are also indicated by closed and open diamonds, respectively.