Fig. 1. The complete nucleotide and deduced amino acid sequence of crayfish Procambarus clarkii antennal gland (kidney) epithelial Ca²⁺ channel (ECaC) cDNA (GenBank accession number AY452713). Nucleotides and amino acids are numbered to the right of the sequence. The start and stop codons are indicated in bold letters. Transmembrane domains and putative pore-forming region are in bold and underlined. The gray boxes indicate the primers used for real-time PCR, RT-PCR and the probe used for in situ hybridization.