Fig. 5. Uncoupling protein (UCP) involvement in antioxidant protection of aconitase activity in oyster mitochondria. (A) GDP (3.5 mmol l^-1) was incubated with intact mitochondria and either removed by washing in nucleotide-free buffer (+wash) or left in the assay medium (-wash) prior to the determination of aconitase activity. ^*Value significantly different from the respective controls (P<0.05). (B) Mitochondria were incubated with GDP (3.5 mmol l^-1), oleate (5 µmol l^-1) and/or Cd (200 µmol l^-1) and washed to remove the additives prior to the measurements of aconitase activity. ^*Values significantly different from the respective controls (no Cd; P<0.05). ^aValue significantly different from the Cd only value at the respective temperature (P<0.05). Values are means ± s.e.m.; N=3 (A), N=5 (B).