Fig. 9. SDS-PAGE of yolk granule (YG) fractions and vitellin (VT) immunolocalization. (A) SDS-PAGE of the different YG fractions. Arrows indicate the four apoproteins in SYGs, LYGs and total egg homogenate (EH); molecular mass is given on the left. (B-E) Vitellin immunolocalization in R. prolixus eggs. Vitellins were present in both LYGs and SYGs during day 0 (B,C) and day 3 (D,E) of embryogenesis. The chorion (ch) was shown to be auto-fluorescent in control groups (data not shown). (B,D) DIC images; (C,E) corresponding fluorescence images. Scale bars, 50 µm.