Fig. 8. Activation of p44ERK by serum (FBS) is dependent on Raf-1, but activation of p44ERK during recovery is independent of Raf-1. (A) Western blot analysis (top) and quantification (bottom) of phospho-p44ERK after serum stimulation of serum-starved cells, in the absence and presence of 10 µmol l^–1 Raf-1 inhibitor, RKI. (B) Western blot analysis (top) and quantification (bottom) of the effect of 10 µmol l^–1 Raf-1 inhibitor on p44ERK phosphorylation during chemical anoxia and recovery (A/R) (N=3, *P<0.05).