Fig. 4. Activation of p44ERK during recovery is MEK dependent. RTHDF cells were challenged with chemical anoxia (A) and recovery (R) in the absence and presence of 10 µmol l^–1 of the MEK1/2 inhibitor PD98059 in the recovery medium. A representative western blot and quantification of band intensity is shown, demonstrating the inhibitory effect of 10 µmol l^–1 PD98059 (N=3, *P<0.05).