Fig. 1. (A,B) Confocal fluorescence images of a live salivary gland incubated with
30 µmol l1 HAF in PS. The optical section plane through
the gland tubule is indicated (upper right of each image). The broken lines in
B indicate the luminal surface of the salivary gland, as deduced from
background fluorescence of the cells. HAF staining is present on the basal
infoldings (broad arrows) and on the lateral domain of the plasma membrane
(arrowheads). Note that staining ends abruptly halfway along the lateral
domain, indicating that HAF does not diffuse across septate junctions. (C) The
structural organization of the secretory portion of the salivary gland.
Asterisks, lumen of the gland; arrowhead, septate junction; long arrow, apical
infoldings (=canaliculi); broad arrow, basal labyrinth. Bar, 20 µm.