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Figure 6


Fig. 6. Relative expression of (A) COX2, (B) CFTR, (C) NKCC1 and (D) NKA1 mRNA was measured by quantitative real-time PCR in gills following acute transfer from freshwater (FW) to seawater (SW; solid lines) or from seawater to freshwater (broken lines). To adjust for different starting expression levels between the two salinities, the relative expression values of the freshwater to seawater series was standardized to the relative chronic salinity means of Fig. 5. Values are means ± s.e.m., N=5 or 6, *P<0.05 for FW to SW, {dagger}P<0.05 for SW to FW.