Fig. 3. SDS-PAGE (12.5%) of the purified stiffening protein (H-tensilin). (A) H-tensilin was run in the presence (left lane) and in the absence (right lane) of 0.1 mol l^–1 dithiothreitol (DTT). Total protein of 0.5 µg was loaded in each lane. (B) Sugar chain detection. Alpha 1 acid glycoprotein (AGP; positive control; first and third lane) and the purified H-tensilin (second and fourth lane) were run on a gel under reducing condition, and the proteins were electroblotted on a PVDF membrane and stained with Coomassie Blue (first and second lane) or with G. P. Sensor stain (third and fourth lane). The same amount of protein was loaded in the first and third lane and in the second and fourth lane. Positions of molecular mass markers and dye front (DF) apply to both A and B.