Fig. 8. X-ray microanalysis of the AgNO₃-stained area on an embryo of Hemigrapsus sexdentatus at stage 4. (A) Secondary electron image. (B) Corresponding mapping of the energy peak for silver atoms showing its localisation over the deposit on the inner membrane. (C) Energy spectrogram showing Ag and Cl atoms are major constituents of the material in the patch (the Au peak is from the sputter coat).