Fig. 2. Results of Experiment 1. Bees trained at units 5 and 10 and then tested in either (A) the training tunnel or (B) a fresh tunnel devoid of scent. Different groups of bees were used in each condition. Bees tested in A were more likely to initiate search (first U-turns; Kolmogorov–Smirnov test, P<0.001) closer to the longer site than bees tested in B. Training consisted of blocks in which bees were either rewarded in the sequence short, long, short, long or vice versa (the sequence alternated hourly). Data from each condition were collected directly following training to either the short or long site. We found no effect of the most recent training location (i.e. no recency effect of memory) in either condition. Black bars, first U-turns; grey bars, second U-turns; coloured lines, search distributions; inverted triangles, training locations; n, flight number.