Fig. 7. Immunohistochemical localisation of phospho-p38-MAPK (A–C) and phospho-HSP27 (D–F) in the ventricle of isolated amphibian heart perfused with Tris–Tyrode's perfusion buffers of various pHs: a normal (control heart) (A,D) or alkaline [(B,E), pH 8.5 or (C,F), pH 9.5] for 2 min. Cryosections were incubated with a phosphospecific anti-p38-MAPK (1:200 dilution) or a phosphospecific anti-HSP27 (1:200 dilution) antibody and processed as described in Materials and methods. Immunolocalisation deposits were visualized with Fast Red chromogen. Representative photographs of three independent experiments are shown. Green arrows indicate the perinuclear localisation. Scale bars, 20 µm.