(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.
Fig. 6. Phosphorylation of HSP27 induced by extracellular alkalosis (pH 8.5) and
effect of the p38-MAPK specific inhibitor SB203580. (A) Protein (100 µg)
from hearts perfused without (Co) or with a Tris–Tyrode's buffer of pH
8.5 for 2 min in the presence or absence of 1 µmol l–1
SB203580 was used to perform western blot analysis with antibodies specific
for the phosphorylated HSP27 (top) or actin (bottom). (B) Densitometric
analysis of phospho-HSP27 by laser scanning. Values are means ± s.e.m.
for three independent experiments. *P<0.05 vs control
value.
