Fig. 7. Spontaneous beating of basal region dissected flagella. A head-attached, reactivated spermatozoon was treated with PRODAN-UV at the basal region of flagellum. Then, the PRODAN-UV treated basal region (between the arrows) was dissected out by a microglass needle. The dissected flagellum continued to show sinusoidal wave movement without a head and base of flagellum. This experiment was repeated more than 20 times. ATP concentration was 15 µmol l^–1. Bar, 10 µm. (A) Micrograph 1, the inhibited reactivated flagellum before its head was dissected out. Micrograph 2, the process of dissection. Micrograph 3–7, phase-contrast micrographs of the reactivated flagellum whose basal region (between two arrows) was PRODAN-UV treated and dissected out. Each micrograph represents 1/30 s. (B) Superimposed shear curves of the panels in A.