Fig. 4. Augmentation contributes to the muscle response during gastric mill rhythms recorded in vitro and in vivo. (A) Top: extracellular recording of the lateral gastric nerve (lgn) showing the activity of LG during a gastric mill rhythm that was elicited by dpon stimulation in the isolated nervous system (in vitro). Bottom: extracellular recording of lgn during a gastric mill rhythm that was elicited by ion stimulation (20 Hz) in the isolated nervous system. (B) Extracellular recordings of the lateral ventricular nerve (lvn) in intact animals (in vivo). The recordings show the activity of the lateral pyloric (LP), pyloric dilator (PD) and LG motor neurons. Three different gastric mill rhythms are shown (weak, intermediate, strong). (C) Intracellular recordings of gm6 muscle showing its response to LG stimulation with standardized in vitro rhythms. Left: ion elicited gastric mill rhythm. Right: dpon elicited gastric mill rhythm. In each panel the first and the tenth stimulus trains are shown. Dotted lines indicate the difference in amplitude of the first EJP and the peak depolarization between first and tenth stimulus train. Please note that amplitude scaling is different for the different types of stimulation. (D) Intracellular recordings of gm6 muscle during stimulation with standardized in vivo gastric mill rhythms. Left: weak rhythm, middle: intermediate rhythm, right: strong rhythm. In each panel the first and the tenth stimulus train are shown. Dotted lines indicate the difference in amplitude of the first EJP and the peak depolarization between first and tenth stimulus train.