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Figure 4


Fig. 4. Immunoblot analysis of urea transporter (UT) protein expressed in the kidney and urinary bladder of the marine toad. The affinity-purified Bufo UT antibody recognized a single band of 52 kDa in extracts of the kidney (lane 1) and urinary bladder (lane 2), but not of heart (lane 3) and liver (lane 4) on negative control tissues. These bands disappeared after preabsorption with the synthetic immunogen (kidney, lane 5; urinary bladder, lane 6).