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Figure 6


Fig. 6. Force–velocity relationships in frog sartorius muscle taken from (4°C) air-access control frogs, normoxic cold-submerged frogs (12–16 weeks in air equilibrated water) and hypoxic cold-submerged frogs (12–16 weeks in 60 mmHg O2) determined using isovelocity contractions (see Appendix for experimental details). Muscle force (P) is normalised to maximal isometric force (P0). Velocity (V) is in muscle lengths (L) s–1. The lines are hyperbolic-linear fits for each treatment of the form V=B(1–P/P0)/(A+P/P0)+C(1–P/P0) (Marsh and Bennett, 1986), calculated using the mean values for the constants A, B and C determined for each muscle for each group. Maximum shortening velocity was significantly different for the three groups (one-way ANOVA: F1,8=4.47, P=0.05). The curvature of the force–velocity relationship (F1,8=0.745, P=0.51) and maximum power output (F1,8=0.832, P=0.47) were not significantly different between the three groups. (G. N. Askew and R. G. Boutilier, unpublished observations.)