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Figure 3


Fig. 3. Cold temperature induces heat shock proteins. (A) Hsp90 amounts in normoxic fish at 26°C, 18°C or 8°C. Results are shown as fold increase compared to levels in normoxic fish at 26°C and are means ± s.e.m. of 5–10 fish. Asterisks denote significant (P<0.001) differences to fish at 26°C (ANOVA and Dunnet's). (B) Hsp70 amounts in normoxic fish at 26°C, 18°C or 8°C. Results are shown as fold increase compared to levels in normoxic fish at 26°C and are means ± s.e.m. of 5–10 fish. Asterisks denote significant differences to fish at 26°C (ANOVA and Dunnet's, P<0.001). (C) Interaction of Hsp90 and Hsp70 with HIF-1{alpha} in crucian carp. Nuclear extracts from the liver of normoxic crucian carp were immunoprecipitated (IP) with Hsp90 antibody, thereafter HIF-1{alpha}, Hsp70 and Hsp90 were detected from the precipitates by immunoblot analysis.