Fig. 1. Plasma glycerol levels in rainbow smelt subjected to a controlled decrease in temperature during three separate experiments: (A) March 2002; (B) December 2002; (C) March 2003. Days following the temperature transition are shown in parentheses. Values are means ± s.e.m. In March 2002 and December 2002 experiments N=6 at each temperature with the exception of December 2002, –0.5°C where N=12. In the March 2003 experiment N=12 for all points except temperatures of 1.5°C where N=10 and 0°C where N=11. Different letters indicate significant differences between two temperatures.