Fig. 7. Visualization of cyprid cement secretion under differential interference contrast (DIC) optics. A cyprid larva was immobilized in agarose and observed in a coverslip chamber using an inverted microscope using DIC optics. The montage shows a series of images separated by 10 s intervals. Images are arranged starting at the top and going left to right. Note the appearance of a vacuole that seems to grow larger with time (arrow). Note the increase in size of the cement sac between frames 1 and 12. See supplemental material for a movie sequence of the original data at http://vivaldi.zool.gu.se/film/Movie-2-sm.mov and http://vivaldi.zool.gu.se/film/Movie-2-sm.avi.