Fig. 4. Effect of hypercapnic acidosis on calcium transient (Ca_iT). (A) Top, a typical continuous recording of myocyte cell length during hypercapnic acidosis. Below, actual tracings of the individual twitch contractions and the Fura-2 fluorescence transients at the times indicated by letters a–c on the continuous chart. (B) Overall results of the effect of acidosis on Ca_iT, shortening and relaxation time of both parameters. The results indicate that during the recovery there is an increase in diastolic and peak systolic [Ca²⁺]_i with a prolongation of the Ca_iT (results from five different myocytes). *P<0.05 vs values before hypercapnia.